Nasal vaccination of beta7 integrin-deficient mice retains elevated IgA immunity

Understanding the migration of lymphocytes to nonintestinal mucosal sites is fundamental to developing mucosal vaccination strategies. Studies have shown that nasal and oral immunization with cholera toxin (CT) stimulates, in addition to α4β7+, the induction of αE (CD103)β7+ B cells. To determine the extent to which αE‐associated β7 contributes to antigen (Ag)‐specific IgA responses in the upper respiratory tract, nasal CT vaccination was performed in wild‐type (wt) and β7‐/‐ mice. At 16 days post‐primary immunization, upper respiratory tract IgA responses were greater in β7‐/‐ mice than in wt mice. IgA induction by distal β7‐/‐ Peyer's patches (PPs), mesenteric LNs (MLNs), and small intestinal lamina propia (iLP) was minimal, in contrast to elevated gut IgA responses in wt mice. By 42 days post‐primary immunization, β7‐/‐ gut IgA responses were restored, and upper respiratory tract Ag‐specific IgA responses were equivalent to those of wt mice. Examination of homing receptor expression and cell‐sorting experiments revealed that β7‐/‐ mice have increased usage of β1 and αE integrins by upper respiratory tract B cells suggesting that alternative integrins can facilitate lymphocyte migration to the upper respiratory tract, especially in the absence of β7.

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