Integrating genomic resources to present full gene and putative promoter capture probe sets for bread wheat
- Gardiner, Laura-Jayne [ Earlham Institute, Norwich, UK ]
- Brabbs, Thomas [ Earlham Institute, Norwich, UK ]
- Akhunov, Alina [ Department of Plant Pathology, Kansas State University, Manhattan, KS ]
- Jordan, Katherine [ Department of Plant Pathology, Kansas State University, Manhattan, KS ]
- Budak, Hikmet [ Montana State University: Plant Sciences & Plant Pathology ]
- Richmond, Todd [ Roche Sequencing Solutions, Madison, WI ]
- Singh, Sukhwinder [ CIMMYT, Ciudad Obregon, Mexico ]
- Catchpole, Leah [ Earlham Institute, Norwich, UK ]
- Akhunov, Eduard [ Department of Plant Pathology, Kansas State University, Manhattan, KS ]
- Hall, Anthony [ Earlham Institute, Norwich, UK ]
Whole-genome shotgun resequencing of wheat is expensive because of its large, repetitive genome. Moreover, sequence data can fail to map uniquely to the reference genome, making it difficult to unambiguously assign variation. Resequencing using target capture enables sequencing of large numbers of individuals at high coverage to reliably identify variants associated with important agronomic traits. Previous studies have implemented complementary DNA/exon or gene-based probe sets in which the promoter and intron sequence is largely missing alongside newly characterized genes from the recent improved reference sequences.