Evidence for persistence of the SHIV reservoir early after MHC haploidentical hematopoietic stem cell transplantation
- Colonna, Lucrezia [ Seattle Childrens Res Inst, Ben Towne Ctr Childhood Canc Res, 1100 Olive Way,Suite 100, Seattle, WA 98101 USA ] [ Univ Washington, Dept Pediat, Box 3563201959,NE Pacific St, Seattle, WA 98195 USA ]
- Peterson, Christopher W. [ Fred Hutchinson Canc Res Ctr, Clin Res Div, Stem Cell & Gene Therapy Program, 1100 Fairview Ave N, Seattle, WA 98109 USA ] [ Univ Washington, Dept Med, Box 3564201959,NE Pacific St, Seattle, WA 98195 USA ]
- Schell, John B. [ Seattle Childrens Res Inst, Ben Towne Ctr Childhood Canc Res, 1100 Olive Way,Suite 100, Seattle, WA 98101 USA ] [ Oregon Hlth & Sci Univ, Vaccine & Gene Therapy Inst, 505 NW 185th Ave, Beaverton, OR 97006 USA ]
- Carlson, Judith M. [ Seattle Childrens Res Inst, Ben Towne Ctr Childhood Canc Res, 1100 Olive Way,Suite 100, Seattle, WA 98101 USA Show more ]
- Tkachev, Victor [ Seattle Childrens Res Inst, Ben Towne Ctr Childhood Canc Res, 1100 Olive Way,Suite 100, Seattle, WA 98101 USA Show more ]
- Brown, Melanie [ Seattle Childrens Res Inst, Ben Towne Ctr Childhood Canc Res, 1100 Olive Way,Suite 100, Seattle, WA 98101 USA Show more ]
- Yu, Alison [ Seattle Childrens Res Inst, Ben Towne Ctr Childhood Canc Res, 1100 Olive Way,Suite 100, Seattle, WA 98101 USA Show more ]
- Reddy, Sowmya [ Fred Hutchinson Canc Res Ctr, Clin Res Div, Stem Cell & Gene Therapy Program, 1100 Fairview Ave N, Seattle, WA 98109 USA ]
- Obenza, Willi M. [ Fred Hutchinson Canc Res Ctr, Clin Res Div, Stem Cell & Gene Therapy Program, 1100 Fairview Ave N, Seattle, WA 98109 USA ]
- Nelson, Veronica [ Fred Hutchinson Canc Res Ctr, Clin Res Div, Stem Cell & Gene Therapy Program, 1100 Fairview Ave N, Seattle, WA 98109 USA ]
- Polacino, Patricia S. [ Washington Natl Primate Res Ctr, 1705 NE Pacific St,Box 357330, Seattle, WA 98195 USA ]
- Mack, Heather [ Washington Natl Primate Res Ctr, 1705 NE Pacific St,Box 357330, Seattle, WA 98195 USA ]
- Hu, Shiu-Lok [ Washington Natl Primate Res Ctr, 1705 NE Pacific St,Box 357330, Seattle, WA 98195 USA ] [ Univ Washington, Dept Pharmaceut, Box 3576101959,NE Pacific St, Seattle, WA 98195 USA ]
- Zeleski, Katie [ Seattle Childrens Res Inst, Ben Towne Ctr Childhood Canc Res, 1100 Olive Way,Suite 100, Seattle, WA 98101 USA ]
- Hoffman, Michelle [ Seattle Childrens Res Inst, Ben Towne Ctr Childhood Canc Res, 1100 Olive Way,Suite 100, Seattle, WA 98101 USA ]
- Olvera, Joe [ Washington Natl Primate Res Ctr, 1705 NE Pacific St,Box 357330, Seattle, WA 98195 USA ]
- Furlan, Scott N. [ Seattle Childrens Res Inst, Ben Towne Ctr Childhood Canc Res, 1100 Olive Way,Suite 100, Seattle, WA 98101 USA ] [ Univ Washington, Dept Pediat, Box 3563201959,NE Pacific St, Seattle, WA 98195 USA ]
- Zheng, Hengqi [ Univ Washington, Dept Pediat, Box 3563201959,NE Pacific St, Seattle, WA 98195 USA ] [ Seattle Childrens Res Inst, Ben Towne Ctr Childhood Canc Res, 1100 Olive Way,Suite 100, Seattle, WA 98101 USA ]
- Taraseviciute, Agne [ Seattle Childrens Res Inst, Ben Towne Ctr Childhood Canc Res, 1100 Olive Way,Suite 100, Seattle, WA 98101 USA ] [ Univ Washington, Dept Pediat, Box 3563201959,NE Pacific St, Seattle, WA 98195 USA ]
- Hunt, Daniel J. [ Seattle Childrens Res Inst, Ben Towne Ctr Childhood Canc Res, 1100 Olive Way,Suite 100, Seattle, WA 98101 USA ]
- Betz, Kayla [ Seattle Childrens Res Inst, Ben Towne Ctr Childhood Canc Res, 1100 Olive Way,Suite 100, Seattle, WA 98101 USA ]
- Lane, Jennifer F. [ Montana State University: Animal Resource Center ] [ Washington Natl Primate Res Ctr, 1705 NE Pacific St,Box 357330, Seattle, WA 98195 USA ]
- Vogel, Keith [ Washington Natl Primate Res Ctr, 1705 NE Pacific St,Box 357330, Seattle, WA 98195 USA ]
- Hotchkiss, Charlotte E. [ Washington Natl Primate Res Ctr, 1705 NE Pacific St,Box 357330, Seattle, WA 98195 USA ]
- Moats, Cassie [ Washington Natl Primate Res Ctr, 1705 NE Pacific St,Box 357330, Seattle, WA 98195 USA ]
- Baldassari, Audrey [ Washington Natl Primate Res Ctr, 1705 NE Pacific St,Box 357330, Seattle, WA 98195 USA ]
- Murnane, Robert D. [ Washington Natl Primate Res Ctr, 1705 NE Pacific St,Box 357330, Seattle, WA 98195 USA ]
- English, Christopher [ Washington Natl Primate Res Ctr, 1705 NE Pacific St,Box 357330, Seattle, WA 98195 USA ]
- Astley, Cliff A. [ Washington Natl Primate Res Ctr, 1705 NE Pacific St,Box 357330, Seattle, WA 98195 USA ]
- Wangari, Solomon [ Washington Natl Primate Res Ctr, 1705 NE Pacific St,Box 357330, Seattle, WA 98195 USA ]
- Agricola, Brian [ Washington Natl Primate Res Ctr, 1705 NE Pacific St,Box 357330, Seattle, WA 98195 USA ]
- Ahrens, Joel [ Washington Natl Primate Res Ctr, 1705 NE Pacific St,Box 357330, Seattle, WA 98195 USA ]
- Iwayama, Naoto [ Washington Natl Primate Res Ctr, 1705 NE Pacific St,Box 357330, Seattle, WA 98195 USA ]
- May, Andrew [ Washington Natl Primate Res Ctr, 1705 NE Pacific St,Box 357330, Seattle, WA 98195 USA ]
- Strensland, Laurence [ Univ Washington, Dept Lab Med, Box 3571101959,NE Pacific St, Seattle, WA 98195 USA ]
- Huang, Meei-Li W. [ Univ Washington, Dept Lab Med, Box 3571101959,NE Pacific St, Seattle, WA 98195 USA ]
- Jerome, Keith R. [ Univ Washington, Dept Lab Med, Box 3571101959,NE Pacific St, Seattle, WA 98195 USA ] [ Fred Hutchinson Canc Res Ctr, Vaccine & Infect Dis Div, 1100 Fairview Ave,NMail Stop E5-110, Seattle, WA 98109 USA ]
- Kiem, Hans-Peter [ Fred Hutchinson Canc Res Ctr, Clin Res Div, Stem Cell & Gene Therapy Program, 1100 Fairview Ave N, Seattle, WA 98109 USA ] [ Univ Washington, Dept Med, Box 3564201959,NE Pacific St, Seattle, WA 98195 USA ] [ Univ Washington, Dept Pathol, Box 3574701959,NE Pacific St, Seattle, WA 98195 USA ]
- Kean, Leslie S. [ Seattle Childrens Res Inst, Ben Towne Ctr Childhood Canc Res, 1100 Olive Way,Suite 100, Seattle, WA 98101 USA ] [ Univ Washington, Dept Pediat, Box 3563201959,NE Pacific St, Seattle, WA 98195 USA ] [ Univ Washington, Dept Med, Box 3564201959,NE Pacific St, Seattle, WA 98195 USA ] [ Boston Childrens Hosp, Karp Res Bldg,Room 08215,1 Blackfan Circle, Boston, MA 02115 USA ] [ Dana Farber Canc Inst, Karp Res Bldg,Room 08215,1 Blackfan Circle, Boston, MA 02115 USA ]
Allogeneic transplantation (allo-HCT) has led to the cure of HIV in one individual, raising the question of whether transplantation can eradicate the HIV reservoir. To test this, we here present a model of allo-HCT in SHIV-infected, cART-suppressed nonhuman primates. We infect rhesus macaques with SHIV-1157ipd3N4, suppress them with cART, then transplant them using MHC-haploidentical allogeneic donors during continuous cART. Transplant results in similar to 100% myeloid donor chimerism, and up to 100% T-cell chimerism. Between 9 and 47 days post-transplant, terminal analysis shows that while cell-associated SHIV DNA levels are reduced in the blood and in lymphoid organs post-transplant, the SHIV reservoir persists in multiple organs, including the brain. Sorting of donor-vs.-recipient cells reveals that this reservoir resides in recipient cells. Moreover, tetramer analysis indicates a lack of virusspecific donor immunity post-transplant during continuous cART. These results suggest that early post-transplant, allo-HCT is insufficient for recipient reservoir eradication despite highlevel donor chimerism and GVHD.