Revisiting an agar-based plate method: What the static biofilm method can offer for biofilm research
- Oja, Terhi [ Pharmaceutical Sciences, Department of Biosciences, Abo Akademi University, Turku, Finland ]
- Blomqvist, Brianna [ Pharmaceutical Sciences, Department of Biosciences, Abo Akademi University, Turku, Finland ]
- Buckingham-Meyer, Kelli [ Center for Biofilm Engineering, Montana State University ]
- Goeres, Darla [ Center for Biofilm Engineering, Montana State University ]
- Vuorela, Pia [ Division of Pharmaceutical Biosciences, CDR, Faculty of Pharmacy, University of Helsinki, Finland ]
- Fallarero, Adyary [ Division of Pharmaceutical Biosciences, CDR, Faculty of Pharmacy, University of Helsinki, Finland ]
The development of biofilms in static plates was monitored. Glass coupons were placed on agar covered with filter paper, which was inoculated with suspended bacteria. The viable cell density, biofilms matrix and biomass were quantified. The method is excellent for adhesion and material studies, due to its simplicity and flexibility.